The Charterhouse of Jerez de la Frontera or Charterhouse of Santa María de la Defensión is a monastery in Jerez de la Frontera, Cádiz, Spain. Its architecture is of a Late Gothic style, corresponding to the start of construction in the 15th century, with Baroque aspects dating from the 17th century. The building, completed in the 17th century, has been protected by the Spanish government since the mid-19th century under a heritage listing.